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The experiment began by weighing the pigs in each group on day 28, recording the initial weight of each pig. They were then weighed again on days 42 and 56, while fasting for 12 hours before each weighing. The daily feed intake was recorded, and the incidence of illness, diarrhea, and death was observed and recorded. The average weight, daily weight gain, average daily feed intake, feed conversion ratio (feed consumption/growth), and diarrhea rate (diarrhea rate = the number of days with diarrhea/the total number of days in the experiment. One pig having diarrhea once or more per day is counted as one day of diarrhea. Each pig participating in the experiment for a day is counted as one trial day) were calculated.

During the experiment, 3 piglets with similar weights from each group (12 in total) were selected for peripheral blood lymphocyte transformation test. In addition, 9 pigs with average weight in each group (3 per duplicate group, totaling 36 pigs) were selected, and around 10ml of blood was collected via the anterior vena cava to prepare serum to measure the following indicators:

(1) Total protein, albumin, and globulin content (biuret method, dye binding method)

(2) Serum glutathione peroxidase (GSH-PX) activity (colorimetric method)

(3) Malondialdehyde (MDA) level (colorimetric method)

(4) The content of IL-2 and IL-6 (radioimmunoassay)

At the age of 42 days, the concentration of IL-2 in the blood of group C was the highest, and at the age of 56 days, the concentration of IL-2 in the blood of group C was significantly higher than that of the control group. The concentration of IL-6 in the blood was the highest in group D at 42 and 56 days old, but the difference compared to other groups was not significant.

At the age of 42 days, the T lymphocyte proliferation rate induced by ConA was the highest in group B at 24 and 48 hours, with group D having the lowest rate. The B lymphocyte proliferation rate induced by LPS was consistent with the T lymphocyte proliferation rate induced by ConA. At the age of 56 days, the T lymphocyte proliferation rate induced by ConA was the highest in groups C and D at 24 and 48 hours, and the B lymphocyte proliferation rate induced by LPS was also consistent with the T lymphocyte proliferation rate induced by ConA.

Replacing 3% of plasma protein in the piglet diet with 3.5% or 5% solpro significantly increased IL-2, IL-6, and globulin concentrations in the blood of 56-day-old piglets, and significantly increased the T lymphocyte and B lymphocyte proliferation rate in the blood. This indicates that using 3.5% or 5% solpro can significantly improve piglet immunity compared to using 3% plasma protein in the diet.